abstract：:A family of 2,3-di-O-acyl trehaloses (DAT), previously identified in Mycobacterium fortuitum, was studied by fast-atom bombardment mass spectrometry to establish the combinations of fatty acyl substituents and, hence, to delineate the molecular species there comprised. The mass spectra indicated the possible existence...

journal_title：FEMS microbiology letters

authors： Ariza MA,Valero-Guillén PL

更新日期：1994-06-15 00:00:00

abstract：:The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. ...

journal_title：FEMS microbiology letters

authors： Simpson WJ,Cieplak W,Schrumpf ME,Barbour AG,Schwan TG

更新日期：1994-06-15 00:00:00

abstract：:Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

journal_title：FEMS microbiology letters

authors： Shima S,Yanagi M,Saiki H

更新日期：1994-06-01 00:00:00

abstract：:Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...

journal_title：FEMS microbiology letters

authors： Powell NG,Threlfall EJ,Chart H,Rowe B

更新日期：1994-06-01 00:00:00

abstract：:We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

journal_title：FEMS microbiology letters

authors： Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

更新日期：1994-06-01 00:00:00

abstract：:Crystalline 3-methylaspartase (EC 4.3.1.2) from Escherichia coli strain YG1002 that had been isolated from soil was characterized. The enzyme activity was induced when the organism was grown statically on medium containing (S)-glutamic acid. Its molecular mass is about 84 kDa, and it may be composed of two identical s...

journal_title：FEMS microbiology letters

authors： Asano Y,Kato Y

更新日期：1994-05-15 00:00:00

abstract：:The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

journal_title：FEMS microbiology letters

authors： Schembri MA,Bayly RC,Davies JK

更新日期：1994-05-01 00:00:00

abstract：:The capsular polysaccharide released from the bacterial surface by cell wall turnover during growth exhibited less size heterogeneity and a higher average molecular mass than the polysaccharide extracted from the cell by treatment with lysostaphin or low pH. Treatment of turnover polysaccharide, radiolabelled by growt...

journal_title：FEMS microbiology letters

authors： Havaei SA,Hancock IC

更新日期：1994-05-01 00:00:00

abstract：:The sequence of 1383 nucleotides of the DNA encoding 16S rDNA was determined for strains of human intestinal spirochaetes, comprising an unnamed isolate and "Brachyspira aalborgi" NCTC 11492. A phylogenetic tree was inferred from aligned sequence comparisons between the intestinal spirochaetes, representatives of the ...

journal_title：FEMS microbiology letters

authors： Hookey JV,Barrett SP,Reed CS,Barber P

更新日期：1994-04-15 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

journal_title：FEMS microbiology letters

authors： Marcello A,Loregian A,Palù G,Hirst TR

更新日期：1994-03-15 00:00:00

abstract：:A relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. Acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrome...

journal_title：FEMS microbiology letters

authors： Toth-Allen J,Torzilli AP,Isbister JD

更新日期：1994-03-01 00:00:00

abstract：:Bordetella calmodulin-like protein was purified from culture supernatant fluid of B. pertussis, B. parapertussis and B. bronchiseptica by successive chromatography on hydroxyapatite, Toyopearl HW-50F and QAE-Toyopearl 550C columns. The purified calmodulin-like protein appeared to be homogeneous by SDS-polyacrylamide g...

journal_title：FEMS microbiology letters

authors： Nagai M,Endoh M,Danbara H,Nakase Y

更新日期：1994-02-15 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:Comparisons of growth and surface colonisation of Enterococcus faecium L-forms and their cell-walled forms were undertaken to produce information about their ability to form sessile cells. The growth of L-forms in liquid culture was slower than that of the parent. This was reflected in their longer lag phase and slowe...

journal_title：FEMS microbiology letters

authors： Jass J,Phillips LE,Allan EJ,Costerton JW,Lappin-Scott HM

更新日期：1994-01-15 00:00:00

abstract：:Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

journal_title：FEMS microbiology letters

authors： Scotland SM,Said B,Thomas A,Rowe B

更新日期：1994-01-15 00:00:00

abstract：:A protein kinase from Dictyostelium discoideum which phosphorylates the synthetic peptide, calmodulin-dependent protein kinase substrate (CDPKS, amino acid sequence: PLRRTLSVAA) and is stimulated by Ca2+/calmodulin is described. This is the first report of a protein kinase with these characteristics in D. discoideum. ...

journal_title：FEMS microbiology letters

authors： Dunbar AJ,Wheldrake JF

更新日期：1994-01-01 00:00:00

abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

journal_title：FEMS microbiology letters

authors： Law C,Kavanagh K,Whittaker P

更新日期：1994-01-01 00:00:00

abstract：:The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), cons...

journal_title：FEMS microbiology letters

authors： Lanfranco L,Wyss P,Marzachí C,Bonfante P

更新日期：1993-12-15 00:00:00

abstract：:Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

journal_title：FEMS microbiology letters

authors： Takemoto JY,Yu Y,Stock SD,Miyakawa T

更新日期：1993-12-15 00:00:00

abstract：:The signal peptidase (SPase) SipS of Bacillus subtilis is responsible for the processing of precursors of secreted proteins. It differs from the SPases of Gram-negative bacteria in structure and specificity. To assay the activity of SipS in vitro, two efficient transcription-translation systems for the synthesis of ra...

journal_title：FEMS microbiology letters

authors： Vehmaanperä J,Görner A,Venema G,Bron S,van Dijl JM

更新日期：1993-12-01 00:00:00

abstract：:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

更新日期：1993-11-15 00:00:00

abstract：:The outer membrane proteins of several prominent bacterial pathogens demonstrate substantial variation in their surface antigenic epitopes. To determine if this was also true for Pseudomonas aeruginosa outer membrane protein OprF, gene sequencing of a serotype 5 isolate was performed to permit comparison with the publ...

journal_title：FEMS microbiology letters

authors： Martin NL,Rawling EG,Wong RS,Rosok M,Hancock RE

更新日期：1993-11-01 00:00:00

abstract：:To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

journal_title：FEMS microbiology letters

authors： Akel HM,Sweet C

更新日期：1993-11-01 00:00:00

abstract：:FNR is an iron-binding transcriptional regulator of Escherichia coli which controls the expression of target genes in response to anaerobiosis. The mechanism used by FNR to sense and respond to anaerobiosis is unknown but it is thought to involve iron. In vitro transcription analyses have shown that iron-deficient FNR...

journal_title：FEMS microbiology letters

authors： Green J,Guest JR

更新日期：1993-10-15 00:00:00

abstract：:The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficient mutants of Saccharomyces cerevisiae were enucleated after treatment with cytochalasin B. Removal of nuclei from protruding sphaeroplasts was induced by centrifugation ...

journal_title：FEMS microbiology letters

authors： Salek AT

更新日期：1993-10-01 00:00:00

abstract：:The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

journal_title：FEMS microbiology letters

authors： Alon RN,Gutnick DL

更新日期：1993-09-15 00:00:00

abstract：:A hybridized DNA fragment was cloned as a 7.6-kb fragment from Streptomyces lavendulae KCCS0263 using a 1.9-kb SacI-XbaI DNA fragment from S. cellulosae as a probe. The latter fragment encoded a beta-lactamase which can bind blue dextran. The hybridized region was reduced to a 2.8-kb KpnI-BclI fragment and the nucleot...

journal_title：FEMS microbiology letters

authors： Sendouda A,Urabe H,Ogawara H

更新日期：1993-09-15 00:00:00

abstract：:NadR is the repressor protein that controls the expression of genes for NAD synthesis. It is also believed to be involved in nucleotide transport. Point mutations conferring different phenotypes were localized to six different regions within the nadR gene. That mutations affecting repression and transport all mapped w...

journal_title：FEMS microbiology letters

authors： Foster JW,Penfound T

更新日期：1993-09-01 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00

abstract：:Calcium has been reported to play an important role in regulating the intestinal electrolyte transport via Ca2+/calmodulin (CaM) and/or protein kinase C (PKC) systems. The role of Ca2+, CaM and PKC in the pathogenesis of Campylobacter jejuni-induced fluid accumulation was studied in vivo in ligated rat ileal loops. Ca...

journal_title：FEMS microbiology letters

authors： Kaur R,Ganguly NK,Kumar L,Walia BN

更新日期：1993-08-01 00:00:00

abstract：:We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluste...

journal_title：FEMS microbiology letters

authors： DiRuggiero J,Achenbach LA,Brown SH,Kelly RM,Robb FT

更新日期：1993-08-01 00:00:00

abstract：:An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...

journal_title：FEMS microbiology letters

authors： Sciortino CV Jr

更新日期：1993-07-15 00:00:00

abstract：:Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

journal_title：FEMS microbiology letters

authors： Desmond EP,Flores M

更新日期：1993-07-01 00:00:00

abstract：:A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

journal_title：FEMS microbiology letters

authors： Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

更新日期：1993-06-15 00:00:00

abstract：:Staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. This processing resulted in the production of multiple molecular-mass forms of each enzyme. Cells from early exponential ...

journal_title：FEMS microbiology letters

authors： Neumann VC,Heath HE,LeBlanc PA,Sloan GL

更新日期：1993-06-15 00:00:00

abstract：:Porphyromonas gingivalis produces a trypsin-like enzyme, Protease I, which is thought to be an important virulence determinant of the organism in adult periodontal disease. Protease I is transiently inhibited by physiological inhibitors of human thrombin. The aim of the present work was to establish whether Protease I...

journal_title：FEMS microbiology letters

authors： Curtis MA,Macey M,Slaney JM,Howells GL

更新日期：1993-06-15 00:00:00

abstract：:The PenJ protein of the penicillinase gene (penP) expression system from Bacillus licheniformis is an antirepressor and membrane receptor for beta-lactam antibiotics. A putative beta-lactam antibiotic binding site including Ser402 and Lys405, which are homologous to the conserved sequence for the beta-lactam binding s...

journal_title：FEMS microbiology letters

authors： Takagi M,Ohta T,Johki S,Imanaka T

更新日期：1993-06-01 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00